Part:BBa_I725011:Design

B-hydroxy butyryl coA dehydrogenase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 290
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 745
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence has been optimized for E. Coli and has had its restriction sites removed. A His tag has been added to the C terminus. The stop codon has been changed to TAATAA.

Source

Genomic sequence of Chlostridium acetobutylicum. CAC_2708.